![]() The purpose of the tool is to normalize batch effects in flow cytometeric datasets collected in different batches, based on a similar set of controls run with each batch. Workaround: Remove spaces and special characters from data files and file paths.The CytoNorm algorithm has been developed and implemented as an R package by Sofie Van Gassen PhD, from the Saeys Lab out of the University of Belgium, in Ghent. R based plugins won’t function when files or file paths include spaces or special characters.The plate editor will not always succeed in applying keywords from file to samples.Quad gates aren’t illustrated in ancestry plots.Workaround: Create NxN plots after batching. Batching layouts with NxN plots can cause corruption in workspaces.Header icons will not transfer from machine to machine when sharing workspaces.Closing and re-opening the layouts dialog disables keyboard shortcuts.Workaround: Make sure to use only standard alpha-numeric characters for data and analyses file naming. Non-standard characters can bet entered as file or workspace names (including spaces and plus signs), which can cause problems downstream.Workaround: Only access workspaces associated with your current FlowJo Envoy environment. Error when opening workspaces associated with a different FlowJo Envoy region, than the one currently being used.Clicking “No” to replace a file in FlowJo Envoy workflow will close the browser.Workaround: Update FlowJo to v10.4+ if you need to access analyses associated with FlowJo Envoy data. Workspaces containing FlowJo Envoy data won’t open correctly in older releases.Workaround: Set scaling of parameters prior to compensation. ![]()
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